Table 2.
S. aureus deletion mutants in animal models of infection.
| System | Gene Cluster | Regulation | Strain | Deletion | Gene Contribution to Virulence in Vivo | Mouse Model of Infection | Evaluated District of Infection | Reference |
|---|---|---|---|---|---|---|---|---|
| Heme acquisition | ||||||||
| Sortases | srtA | Constitutive | Newman | ΔsrtA | Yes | C57BL/6, Swiss-Webster (intravenous) | Kidney | [183] |
| isdC-FsrtBisdG | Fur | Newman | ΔsrtA | Yes | NMRI (intra-articular) | Joints, kidney, blood | [184] | |
| Newman, USA300 | ΔsrtA | Yes | BALB/c (intravenous) | Kidney | [150] | |||
| Newman D2C | ΔsrtA | Yes | BALB/c (mammary injection) | Mammary glands | [185] | |||
| Newman | ΔsrtA, ΔsrtA-srtB | Yes | CD-1 (intraperitoneal), | Systemic, joints, | [186] | |||
| NMRI (intravenous), C3H/HeJ (bladder), Sprague-Dawley rats (intravenous) | kidney, heart | |||||||
| Newman | ΔsrtA, ΔsrtA-srtB | Yes | NMRI (intravenous) | Joints, kidney | [187] | |||
| Newman | ΔsrtB | Mild | Swiss-Webster (intravenous) | Kidney | [142] | |||
| Newman | ΔsrtB | Mild | NMRI (intravenous) | Joints, kidney | [187] | |||
| Newman | ΔsrtB | Mild | CD-1 (intraperitoneal), NMRI (intravenous), C3H/HeJ (bladder), Sprague-Dawley rats (intravenous) | Systemic, joints, kidney, heart | [186] | |||
| Isd | isdA | Fur | Newman | ΔisdBH, ΔhtsA-isdE | No | BALB/c (intranasal) | Lung | [188] |
| isdB | Fur | Newman | ΔhtsA-isdE | Yes | BALB/c (retro-orbital) | Lung, heart, kidney | [188] | |
| isdC-FsrtBisdG | Fur | Newman | ΔisdB, ΔisdA | Yes | BALB/c (intravenous) | Kidney | [150] | |
| orfXisdI | Fur | Newman | ΔisdC | Mild | BALB/c (intravenous) | Kidney | [150] | |
| isdH | Fur | Newman | ΔisdH | No | BALB/c (intravenous) | Kidney | [150] | |
| Newman | ΔisdB | Yes | C57BL/6J (retro-orbital) | Heart | [149] | |||
| Newman | ΔisdB | No | C57BL/6J (retro-orbital) | Liver | [149] | |||
| Newman | ΔisdA, ΔisdB, ΔisdC | Yes | BALB/c (retro-orbital) | Kidney | [151] | |||
| Newman | ΔisdH | No | BALB/c (retro-orbital) | Kidney | [151] | |||
| Newman | ΔisdG, ΔisdI, | Yes | BALB/c (retro-orbital) | Heart | [159] | |||
| Newman | ΔisdG-I | No | BALB/c (retro-orbital) | Kidney | [159] | |||
| Newman | ΔisdI | Yes | BALB/c (retro-orbital) | Kidney | [159] | |||
| Newman | ΔisdG, ΔisdG-I | No | BALB/c (retro-orbital) | Liver | [159] | |||
| Newman | ΔisdG, ΔisdI, | Mild | BALB/c (retro-orbital) | Kidney, spleen | [56] | |||
| Newman | ΔisdG-I | Yes | BALB/c (retro-orbital) | Kidney, spleen | [56] | |||
| 8325-4 | ΔisdH | Yes | NMRI (intravenous) | Blood | [165] | |||
| ΔisdB, ΔisdB-isdH | ||||||||
| ΔisdH | ||||||||
| Hss | hssRS | Constitutive, activated by heme | Newman | ΔhssR | Mutation increase virulence | BALB/c (retro-orbital) | Liver | [175] |
| Newman | ΔhssR | No | BALB/c (retro-orbital) | Spleen, kidney | [175] | |||
| Hrt | hrtAB | HssRS | Newman | ΔhrtA | Mutation increase virulence | BALB/c (retro-orbital) | Liver | [175] |
| Newman | ΔhrtA | No | BALB/c (retro-orbital) | Spleen, kidney | [175] | |||
| Endogenous siderophores | ||||||||
| Staphyloferrin A | sfaABC | Fur | Newman | Δsfa | Yes | BALB/c (subcutaneous) | Skin | [88] |
| sfaD | Fur | Newman | Δsfa-sbn | Yes | BALB/c (subcutaneous) | Skin | [88] | |
| htsABC | Fur | |||||||
| MW2 | ΔsfaA | Yes | Swiss-Webster (intravenous) | Kidney | [89] | |||
| Newman | ΔhtsA-isdE | No | BALB/c (intranasal) | Lung | [188] | |||
| Newman | ΔhtsA-isdE | Yes | BALB/c (retro-orbital) | Lung, heart, kidney | [188] | |||
| Newman | ΔhtsB, ΔhtsC | Yes | BALB/c (intravenous) | Kidney, liver | [53] | |||
| Staphyloferrin B | sbnA-I | Fur, SbnI | Newman | ΔsbnE | Yes | Swiss-Webster (intravenous) | Kidney | [90] |
| sirABC | Fur | Newman | Δsbn | No | BALB/c (subcutaneous) | Skin | [99] | |
| MW2 | ΔsbnD | Yes | Swiss-Webster (intravenous) | Kidney | [89] | |||
| Newman | ΔsbnG | No | BALB/c (intravenous) | Heart, kidney, liver | [87] | |||
| Newman | ΔsbnG-citZ | Yes | BALB/c (intravenous) | Heart, kidney, liver | [87] | |||
| Newman | Δsfa-sbn, Δhts-sir, Δsfa-sbn-sst, | Yes | BALB/c (intravenous) | Heart, kidney, liver | [109] | |||
| Δhts-sir-sst | ||||||||
| Xenosiderophores | ||||||||
| Hydroxamate | fhuBGC | Fur | Newman | ΔfhuD2 | Yes | CD1 (intravenous) | Kidney, blood | [110] |
| fhuD1 | Fur | Newman | ΔfhuBGC | Yes | Swiss-Webster (intravenous) | Kidney | [189] | |
| fhuD2 | Fur | |||||||
| Catecholate | sstABCD | Fur | Newman | Δsst | Yes | BALB/c (intravenous) | Heart | [109] |
| Inorganic iron acquisition | ||||||||
| Fep | tatAC | Constitutive | RN1HG | ΔtatAC, Δtat-fep | Yes | BALB/c (intravenous) | Kidney | [170] |
| fepABC | Fur | |||||||