Figure 7.

Atezolizumab increased T cell-mediated cytotoxicity in A549 cells treated with pemetrexed. A549 tumor cells and PBMCs activated with anti-CD3 (1 μg/mL) and anti-CD28 (2 μg/mL) were co-cultured in transwells for 3 days with or without 100 nM pemetrexed. Then PBMC were transferred to contacting co-culture by transferring them from the insert to the corresponding well with a ratio of approximately 5:1 (effector: target cells) for 24 h with or without 200 µg/mL atezolizumab. (A) Images of A549 cells co-cultured with PBMCs in the absence or presence of 100 nM pemetrexed with or without 200 µg/mL atezolizumab. Scale bar: 100 μm. (B) Crystal violet assay *** p < 0.01. Data in (B) are the average of three independent experiments.