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. 2020 Mar 16;21(6):2007. doi: 10.3390/ijms21062007

Figure 3.

Figure 3

Inhibitory effects of L7Gn on NF-κB and MAPK activation. (A and B) RAW 264.7 macrophages were pre-treated with different concentrations of L7Gn (0, 5, 10, 20, and 50 μM) for 2 h, followed by LPS (1 μg/mL) stimulation for 3 min (A) or 15 min (B). Total cell lysates were prepared and immunoblot analysis was performed. The expression of p-IκBα, IκBα (A), p-p38, p38, p-JNK, c-Jun N-terminal kinase (JNK), p-ERK, and ERK (B) was detected using specific antibodies. Expression levels of p-IκBα and IκBα were normalized to GAPDH levels. Levels of phosphorylated MAPKs were normalized to the corresponding total MAPK levels. Results of the quantitative analyses of phosphorylated or total protein levels after normalization are shown (lower panel). Data represent the mean ± S.D. # p < 0.01 relative to the non-treated control group. * p < 0.05 and ** p < 0.01 relative to the non-treated (IκBα in A) or LPS-treated and 5 μM L7Gn-treated group (B and p-IκBα in A).