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. 2020 Mar 16;21(6):2007. doi: 10.3390/ijms21062007

Figure 4.

Figure 4

Effects of L7Gn on LPS-induced phosphorylation of TAK1 and degradation of IRAK1. RAW 264.7 cells were pre-treated with L7Gn for 2 h and then treated with LPS (1 μg/mL) for 3 min. Total proteins were subjected to immunoblot analysis using the indicated specific antibodies. (A) The level of phosphorylated TAK1 was normalized to that of total TAK1. (B) The expression of IRAK1 was normalized to that of GAPDH. Results of the quantitative analyses of phosphorylated or total protein levels after normalization are shown (lower panel). Data represent the mean ± S.D. # p < 0.01 relative to the LPS-untreated control group. # p < 0.01 relative to the LPS-untreated control group. * p < 0.05 and ** p < 0.01 relative to the LPS-treated and 5 μM L7Gn-treated group.