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. 2020 Mar 12;21(6):1930. doi: 10.3390/ijms21061930

Figure 1.

Figure 1

Lipid nanoparticles carrying oncosuppressive miRNAs inhibit melanoma cell proliferation. A375 (A) and M14 (B) melanoma cells were exposed to 30 μg of the indicated LNPs for 72 h: LNP1 (empty), LNP2 (miR-204-5p), LNP3 (miR-199b-5p) or LNP4 (miR-204-5p + miR-199b-5p). Viability was determined through crystal violet staining to obtain % of cell proliferation as compared to LNP1-treated cells. Cellular ATP was tested by luminescence assay to determine metabolic activity in response to LNP1 and LNP4 treatments in A375 (C) and M14 (D) cells. Crystal violet staining and O.D. at 595 nM reading by spectrometer assessed the growth inhibitory effects of LNPs in BRAFi-resistant cells (i.e., A375R (E) and M14R (F)). Cellular ATP measure, performed as described above, was used to test the effects of empty LNPs as compared to untreated A375 and M14 cells ((G), left and right panels).