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LNP4 affects different downstream effectors in response to the upregulation of miR-204-5p and miR-199b-5p. (A,B) A375 cells were treated with LNP1 or LNP4, as described above, and total protein extracts were subjected to Western blot analysis to measure the expression levels of the indicated molecular effectors downstream of miR-204-5p and miR-199b-5p. (C) ELISA-based reading was used to determine the levels of soluble VEGF-A in response to LNPs treatments in A375 cells.
