Table 1.
Characteristics of LNP blank (LNP1) or encapsulating miRNA (LNP1, LNP2 and LNP3). For each formulation, size, polydispersity index (PI), zeta potential (ZP), actual loading and encapsulation efficiency (EE%) were calculated as the mean of the measures obtained at least on three different batches.
| Formulation | miRNA | Diameter (nm) ± SD |
PI ± SD | ZP (mV ± SD) |
Actual Loading (μg miRNA/mg Lipids) |
EE% ± SD |
|---|---|---|---|---|---|---|
| LNP1 | - | 138 ± 9 | 0.19 ± 0.02 | −11 ± 1 | - | - |
| LNP2 | miR-204-5p | 157 ± 3 | 0.17 ± 0.05 | −28 ± 8 | 186 ± 0.005 | 93 ± 3 |
| LNP3 | miR-199b-5p | 160.4 ± 11 | 0.18 ± 0.08 | −18 ± 8 | 199 ± 0.001 | 99 ± 1 |
| LNP4 | miR-204-5p/199b-5p | 167 ± 5 | 0.15 ± 0.05 | −26 ± 9 | 190 ± 0.009 | 95 ± 5 |