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LDA phenotype at damage sites of RNA-specific stained (terbium-citrate; Tb) sections vanish after RNase treatment. Sections of U2OS nuclei irradiated with carbon ions, fixed 1 h after irradiation and stained with Tb (A) show LDAs correlating with 53BP1 signal (B, green). After 24 h treatment with RNase no LDAs are visible (C) at the damage site (D). All scale bars: 1 µm.
