Skip to main content
. 2020 Mar 3;12(3):582. doi: 10.3390/cancers12030582

Figure 4.

Figure 4

Figure 4

Figure 4

Higher levels of DNA damage were observed after treatment with a shorter time interval between ionizing radiation and hyperthermia. (A) Cells in S-phase were excluded to only count the γ-H2AX positive double strand breaks (DSBs) that were radiation-induced. (B) SiHa cells demonstrating the DNA damage using γ-H2AX foci—cells were fixed 24 h after treatments. (C–E) γ-H2AX foci of HPV16+, HPV18+, and HPV-negative cell lines between ionizing radiation (IR; 2 Gy) and hyperthermia (HT) after treatments with different time intervals, different sequences, and various temperatures. Means with standard deviation of at least three replicates are presented, with a minimum of 100 cells per replicate. * p < 0.05, ** p < 0.01, and *** p < 0.001 indicate the difference between 0 and 1, 2, 3, and 4 h time interval between ionizing radiation and hyperthermia, or the opposite order of treatments.