Figure 4.

Haprolid treatment inhibits G1/S transition and induces cell cycle arrest in HCC cells. (A) HCC cells were treated with vehicle (DMSO) or increasing concentrations of haprolid for 48 and 96 h, fixed and stained with propidium iodide (PI) and subjected to flow cytometric analysis. Representative cell cycle histograms are presented (left panel). Cell cycle distribution in various phases is determined and shown (right panel). (B) Cyclin A and B expression levels were measured by Western blotting. Images representative of at least three independent experiments are shown.