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Dual inhibition of Rb/E2F and Akt/mTOR pathways by haprolid. (A and B) Hep3B, Huh-7 and HepG2 cells were treated with increasing concentrations of haprolid or DMSO for 96 h. Cell lysates were analyzed by Western blotting with antibodies against indicated proteins. β-actin is shown as a loading control. Images representative of at least three independent experiments are shown.
