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Receptor quantification and in vitro specificity. (A) HER3 expression was quantified for BxPC-3 (n = 2) and DU145 (n = 2) cells by incubation with [57Co]Co-(HE)3-ZHER3-NOTA until saturation. For the in vitro specificity test in (B) BxPC-3 and (C) DU145 cells, binding to HER3 was inhibited by addition of 50 nM HER3 binding affibody in the blocked groups. Specificity data is presented as the average of three dishes ± SD.
