Figure 7.

Claudin-12 wild type (WT) and knockout (KO) mouse renal mRNA expression of key genes involved in Ca²⁺ transport. (A) Calcium transport mediators: transient receptor potential cation channel subfamily V member 5 (TRPV5), calbindin 1 (Calb1), plasma membrane calcium ATPase 1b (PMCA1b), and calcium sensing receptor (CaSR). (B) Sodium transporters: sodium–potassium–chloride cotransporter 2 (NKCC2), sodium–chloride cotransporter (NCC), sodium-hydrogen exchanger 3 (NHE3), sodium–calcium exchanger (NCX), and epithelial sodium channel (ENaC). (C) Vitamin D related genes: vitamin D receptor (VDR), 25-hydroxyvitamin D 1 alpha hydroxylase (1aOHase), 24-hydroxylase (24aOHase), and klotho. (D) Renal claudins involved in cation transport normalized to 18S as house-keeping gene: claudins 1, 2, 3, 8, 10a, 14, 15, 16, and 19. n = 26 and 35, respectively. *—significant with Benjamini–Hochberg critical value for false discovery rate of 0.05. ° represents values that are above or below 1.5 times the interquartile range.