Figure 1.

CaN inhibition dynamically regulates GLAST expression in mouse hippocampal astrocytes. (A) Glutamate/aspartate transporter (GLAST) protein expression in primary cultures of hippocampal astrocytes from control not-treated (Ctr, NT) Ctr astrocytes treated with FK506 200nM for 1, 2 and 7 days and of cultured astrocytes from mice with an astrocyte-specific deletion of Ca²⁺/calmodulin-activated phosphatase calcineurin (CaN-KO). Data are expressed as mean ± SD, 5 independent cultures were used for each condition. * p < 0.05; ** p < 0.01; *** p < 0.001, one way ANOVA with Tukey post hoc test. (B) Immunofluorescence anti-GLAST images of Ctr-NT, Ctr treated with FK506 200 nM for 7 days and CaN-KO. Green, GLAST; blue, DAPI (4′,6-diamidino-2-phenylindole). Bar, 50 μm. (C) Real-time PCR of GLAST from primary astrocytes from Ctr-NT, Ctr treated with FK506 200 nM for 1, 2, and 7 days, and from astrocyte-specific CaN-KO mice. Values represent mean  ±  SD ΔC(t) of gene/S18 of 6 independent cultures for each condition.