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. 2020 Mar 23;21(6):2228. doi: 10.3390/ijms21062228

Figure 3.

Figure 3

Mimic miR-34a increases cell-death in human IPF-lung fibroblasts Work flow; fibrotic-lung myofibroblasts (ATCC-CCL-191) were transfected with mimic miR34a (5 nM, 72h, Trans-iT) vs. control (NC) and then co-cultured with Jurkat T cells. (A) Microscope images with trypan-blue exclusion (arrows denote myofibroblasts; triangles, lymphocytes) (B), graphical presentation of cell survival percentage and (C), Graphical presentation of cleaved/uncleaved caspase-3 ODs (Upper panel) and Wb (insert). Representative results from three independent experiments. Error bars represent mean ± SD; circles represent individual data points. * p < 0.01–0.05.