Figure 4.

Kinetics profile of miR-34a, in Bleomycin-treated mice shows reduction at times of fibrosis evolution and upsurge to normal levels at resolution, and an inverse correlation with FLIP. C57BL/6 wild-type mice were exposed to Bleomycin (0.03 mU/mice) or control-saline, sacrificed 14, 56 days post Bleomycin-OA, and myofibroblasts were isolated. (A) Total RNA was extracted and qPCR miR-34a expression levels determined (calculated as 2^−∆∆Ct). (B) Myofibroblast cell lysates were obtained to assess FLIP in Wb using anti-FLIP mAb. OD ratios, normalized to β-actin at each time point, are presented. One representative of four experiments with similar results; n = 5–6 for each time point; error bars represent mean ± SD; circles represent individual data points. * p < 0.001.