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Native 7% electrophoretic mobility shift assay (EMSA) of A. (a) and N.A. (b) V7t1 and covalent V7t1 dimers incubated in the presence (+) or absence (−) of VEGF165. GelGreen- and Coomassie-stained gels (left and right, respectively). 30 pmol of each aptamer were incubated with 40 pmol of the protein in a final volume of 9 μL in the selected HEPES/Na+ buffer, thus obtaining a final 1:1.3 oligo/protein ratio. Gels were run at constant 45 V for 2.3 h at r.t. in TAE 1X buffer.
