Table 2.
Prediction of relative abundance of the G4 topologies adopted by N.A. and A. samples.
| Prediction of G4 Topologies Relative Abundance from SVD Analysis of CD Spectra | |||
|---|---|---|---|
| HEPES/Na+ | |||
| Parallel (%) | Hybrid (%) | Antiparallel (%) | |
| N.A. V7t1 | 100 | 1 | 0 |
| A. V7t1 | 36 | 0 | 63 |
| N.A. bisV7t1T7 | 72 | 7 | 20 |
| A. bisV7t1T7 | 32 | 0 | 67 |
| N.A. bisV7t1HEG2 | 78 | 0 | 23 |
| A. bisV7t1HEG2 | 34 | 0 | 65 |
| N.A. bisV7t1TEG2D | 100 | 1 | 0 |
| A. bisV7t1TEG2D | 75 | 26 | 0 |
| TRIS/K+ | |||
| N.A. V7t1 | 100 | 1 | 0 |
| A. V7t1 | 92 | 2 | 6 |
| N.A. bisV7t1T7 | 97 | 4 | 0 |
| A. bisV7t1T7 | 45 | 38 | 16 |
| N.A. bisV7t1HEG2 | 100 | 1 | 0 |
| A. bisV7t1HEG2 | 100 | 1 | 0 |
| N.A. bisV7t1TEG2D | 100 | 1 | 0 |
| A. bisV7t1TEG2D | 100 | 1 | 0 |
The prediction for V7t1 and its covalent dimers was obtained by singular value decomposition (SVD) analysis of the CD spectra recorded in both the selected HEPES/Na+ and TRIS/K+ buffer solutions, performed by exploiting the software developed by del Villar-Guerra et al. [60]. Deviations from 100% (±1%) are due to significant digits approximation of the values originally obtained by the simulations.