Figure 3.

Effects of bradykinin on phosphorylation of mitogen-activated protein kinase kinase (MEK)1 and extracellular signal-regulated kinase (ERK1)/2 in human malignant glioblastoma cells. Human U87 MG glioblastoma cells were treated with 100 nM bradykinin for 0.5, 1, and 3 h. Levels of phosphorylated (p)-MEK1and p-ERK1/2 were immunodetected (A,C, top panels). Amounts of MEK1 and ERK1 were analyzed as the internal controls (bottom panels). These protein bands were quantified and statistically analyzed (B,D). Each value represents the mean ±standard deviation (SD) for n = 9. An asterisk (*) indicates that a value significantly (p < 0.05) differed from the respective control. Representative immunoblots are shown.