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. 2020 Mar 13;12(3):667. doi: 10.3390/cancers12030667

Table 1.

Effects of bradykinin on survival, intracellular calcium concentrations, AQP4 mRNA expression, wound healing, and invasion of murine GL261 glioblastoma cells.

Cell Activities Control Bradykinin
Cell survival, cell numbers × 103 46 ± 9 43 ± 10
[Ca2+]i, fluorescent intensities × 103 0.8 ± 0.2 16.2 ± 3.5 *
AQP4 mRNA, quantity of relative expression 1 ± 0.2 3.2 ± 0.7 *
Wound healing, % of area 57 ± 11 23 ± 5 *
Cell invasion, cell numbers 49 ± 9 578 ± 99 *

Murine GL261 glioma cells were treated with bradykinin for different intervals. Cell survival was determined using a trypan blue exclusion assay. Levels of intracellular calcium [Ca2+]i influx were analyzed using confocal microscopy. AQP4 mRNA was quantified with a real-time PCR. Migration of GL261 cells was examined using wound healing and matrigel-based migration assays. An asterisk (*) indicate that a value significantly (p < 0.05) differed from the respective control.