Table 1.
Cell Activities | Control | Bradykinin |
---|---|---|
Cell survival, cell numbers × 103 | 46 ± 9 | 43 ± 10 |
[Ca2+]i, fluorescent intensities × 103 | 0.8 ± 0.2 | 16.2 ± 3.5 * |
AQP4 mRNA, quantity of relative expression | 1 ± 0.2 | 3.2 ± 0.7 * |
Wound healing, % of area | 57 ± 11 | 23 ± 5 * |
Cell invasion, cell numbers | 49 ± 9 | 578 ± 99 * |
Murine GL261 glioma cells were treated with bradykinin for different intervals. Cell survival was determined using a trypan blue exclusion assay. Levels of intracellular calcium [Ca2+]i influx were analyzed using confocal microscopy. AQP4 mRNA was quantified with a real-time PCR. Migration of GL261 cells was examined using wound healing and matrigel-based migration assays. An asterisk (*) indicate that a value significantly (p < 0.05) differed from the respective control.