. 2020 Mar 23;21(6):2208. doi: 10.3390/ijms21062208

Table 1.

Application examples of the investigated assays in previous studies. ND, not determined; MD, metal-dependence; R, References.

Assay	Application	Enzyme	Species	GT Family	Fold	Mechanism	MD	R
pH-Sensitive	-Screening GTs -Saturation mutagenesis library	GTB	H. sapiens	6	GT-A	Retaining	Mn²⁺	[25]
		GTA	H. sapiens	6	GT-A	Retaining	Mn²⁺	[25]
		GalT1	H. sapiens	7	GT-A	Inverting	Mn²⁺	[44]
		LgtB	N. meningitidis	25	ND	Inverting	Mn²⁺	[44]
		HP0826	H. pylori	25	ND	Inverting	ND	[44]
Nucleotide- Glo^TM (UDP, GDP, UMP)	-Sugar-nucleotide donor specificity -Screening of GT inhibitors	POMGNT1	H. sapiens	13	GT-A	Inverting	Mn²⁺	[45]
		B4GAT1	H. sapiens	49	ND	Inverting	Mn²⁺	[45]
		SpGtfA (OGT)	S. pneumonia	41	GT-B	Inverting	ND	[36,45]
		DdAgtA	D. discoideum	77	ND	Retaining	Mn²⁺	[45]
		POGLUT1	H. sapiens	90	ND	Inverting	ND	[45]
		β4 Gal-T1	Bos taurus	7	GT-A	Inverting	Mn²⁺	[36,45]
		LARGE1	H. sapiens	49/8	ND/GT-A	Inverting/Retaining	Mn²⁺	[45]
		PglC	C. jejuni	4	GT-B	Retaining	Mn²⁺/Mg²⁺	[46]
		PglC	H. pullorum	4	GT-B	Retaining	Mn²⁺/Mg²⁺	[46]
		WecA	T. maritima	4	GT-B	Retaining	Mn²⁺/Mg²⁺	[46]
		UGT1A1	H. sapiens	1	GT-B	Inverting	ND	[36]
		GTB	H. sapiens	6	GT-A	Retaining	Mn²⁺	[36]
		GALNT1	H. sapiens	27	GT-A	Retaining	Mn²⁺	[36]
		ST6GAL1	H. sapiens	29	ND	Inverting	ND	[37]
		UGT2B17	H. sapiens	1	GT-B	Inverting	ND	[36]
		FUT2	H. sapiens	11	ND	Inverting	ND	[36]
		FUT3	H. sapiens	10	GT-B	Inverting	ND	[36]
		FUT7	H. sapiens	10	GT-B	Inverting	ND	[36]
		IRX10-L	A. thaliana	47	GT-B	Inverting	ND	[47]
		AtFUT1	A. thaliana	37	GT.B	Inverting	ND	[48]
PO₄ GT Assay	-Kinetic analyses	TcdB	C. difficile	44	ND	Retaining	ND	[39]
		KTELC1	H. sapiens	90	ND	Inverting	ND	[39]
		ST6GAL1	H. sapiens	29	ND	Inverting	ND	[39]
UDP² TR-FRET	-Discovery of GT inhibitors	GALNT3	H. sapiens	27	GT-A	Retaining	Mn²⁺	[41]