Figure 5.

Effect of H₂O₂ on integrin α7-dependent migration. Migration on laminin-111 of HT1080 cells expressing integrin α7wt (a) or α7hi (b) was monitored on CIM-plates in an xCELLigence DP device, in the absence and presence of 10 µM H₂O₂. The experiments were carried out in Tyrode’s solution and in the presence of 2 µg/mL GoH3. Means ± SD. of Δcell index values, measured in 5 min intervals, from at least four independent measurements are shown. The Holm-Sidak method revealed a significant difference (p < 0.05) for α7wt-, but not α7hi-transfectants, after the first 60 min of H₂O₂ treatment. (c) The average migration rates between 30 and 60 min after migration start are shown from one of three experiments with quadruplet determination with means ± SD. Significance level: ** p ≤ 0.01. (d) Relative ΔΔcell index values were calculated at each time point for HT1080 cells expressing α7wt or α7hi to compare H₂O₂-treated and nontreated samples. Means ± SD are shown (n = 4 for α7wt and n = 6 for α7hi).