Figure A2.

Mass-spectrometric analysis of proteins trapped by Trx1-trap mutant. The Cys-X-X-Ser mutant of Trx1 was applied in an intermediate trapping approach to trap Trx1 substrates on α7wt-HT1080 cells. The trapped proteins were analyzed after tryptic digestion, using liquid chromatography coupled with mass spectrometry. Among the trapped proteins, integrin α7 was identified with a MaxQuant score of 22.9 with three individual peptides (marked in red). All three peptides were identified at a 2+ charge state, with individual MaxQuant scores of 76.5 (AIDLEQPNCADGR, PEP 1.1 E-7), 44.9 (LIPEVVLSGER, PEP 0.0027) and 36.7 (SEELSFVAGAPR, PEP 0.0055). The two cysteines of the hinge motif are highlighted with black boxes.