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Demethylating agent 5-aza-2’deoxycytidine increases CXCR4 transcript abundance. RT-PCR results display a decreased normalized Ct value (average) and therefore reflects the increased CXCR4 mRNA expression in colorectal cancer cell lines (a) HCT116 and (b) HT29 after treatment with 5 µM concentration of demethylating agent 5-aza-2’-deoxycytidine.
