Skip to main content
. 2020 Mar 2;12(3):576. doi: 10.3390/cancers12030576

Figure 2.

Figure 2

Decreased level of peroxisomal Nudt7 is observed in KrasG12D CRC dysregulation. (A) Expression level of Nudt7 in CRC tumors using GSE8671 dataset and presented as the fold change compared with normal. (B) Expression level of Nudt7 in tumors or adjacent non-tumor areas of KrasG12D and KrasWT CRC. Rn18s was used as an endogenous control (n = 3). (C) NUDT7 expression was analyzed via immunohistochemistry using tamoxifen-inducible Villin-CreERT2; Apcf/f; Trp53f/f; tetO-LSL-KrasG12D mice (KrasOFF and KrasON) and human patients (KrasWT and KrasG12D). Positive cells were counted for every 100 cells in 3 different fields at 200× magnification. Results are representative of at least 3 independent experiments. Scale bars: 100 μm. Values are means + SD. An unpaired Student’s t-test was used for statistical analysis. *** p < 0.001, **** p < 0.0001.