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. 2020 Mar 2;12(3):576. doi: 10.3390/cancers12030576

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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AOM/DSS (azoxymethane/dextran sulfate sodium)-treated Nudt7^−/− mice increases the possibility of CRC development. (A) Mice were treated with AOM/DSS and polyp number was counted. Scale bars: 2 cm. (B,C) Immunohistochemistry with hematoxylin and eosin (H&E), Ki67, F4/80, LY6G, NUDT7, ACOX1, and SCD1 (n = 4) and positive cells were counted for every 100 cells in 3 different fields at 100× magnification in Nudt7^+/+ and Nudt7^−/− colons treated with AOM/DSS. Results are representative of at least 3 independent experiments. Scale bars: 200 μm. (D,E) Transcriptional levels of proliferation and inflammatory marker genes and Kras^G12D target genes in Nudt7^+/+ and Nudt7^−/− colons treated with AOM/DSS. Rn18s was used as an endogenous control. Results are representative of at least 3 independent experiments. Values are means + SD. An unpaired Student’s t-test was used for statistical analysis. * p ≤ 0.01, ** p < 0.01, *** p < 0.01, **** p < 0.0001.