Figure 4.

NAC reduced severity of hepatic steatosis and adipose tissue inflammation in HFD-fed offspring male mice. NAC was given to females during pregnancy and lactation. HFD was given to offspring from the age of 6 weeks. Mice were killed at age 16 weeks and H&E staining of liver (A) and adipose tissue (E) was performed. Severity of steatosis was graded using steatosis score, as described in Methods (B). Visceral adipose tissue weight was measured (C) and adipocyte diameter was measured (D). C/STD: STD-fed offspring of control mice, C/HFD: HFD-fed offspring of control mice, NAC/HFD: HFD-fed offspring of NAC-treated mice. The results are presented as mean ± SE (n ≥ 4), ** p < 0.005, **** p < 0.005 in one-way Anova followed by Tukey’s post-test.