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. 2020 Mar 24;12(3):767. doi: 10.3390/cancers12030767

Figure 2.

Figure 2

Expression of BRAF V600E mutation in melanoma tissue. With routine immunohistochemistry, the distribution of BRAF V600E mutation can be easily detected in melanoma tissue by a standardized colorimetric assay. Mutated BRAF V600E proteins react with HRP-DAB (peroxidase conversion of diaminobenzidine) (a) Homogeneity of the BRAF V600E protein expression. (b) Heterogeneity of the BRAF V600E protein expression. The peripheral and focal central regions of show a pronounced expression of the mutated protein. In areas with mild V600E expression, there is marked intercellular heterogeneity. BRAF V600E IHC, clone VE1, HIER (heat-induced epitope retrieval) pH = 9; OM 112 ×.