Figure 1.

High-throughput siRNA screening in the mucinous epithelial ovarian cancer (mEOC) cell line MCAS. (A) Summary of the effects of downregulation on each member of the 719-in-size target pool of the siRNA library. Data are plotted as ratios of transfected siRNA/negative control siRNA mean absorbance values (T/C) and represent the means of the independent siRNA screenings done. The dashed line selected 17 siRNA with a T/C ≤ 0.6. The arrow underlines the T/C value of PLK1. (B) Validation of siRNA screening. MCAS cell survival after 96 h of transfection using specific esiRNA against positive hits identified in the screening.