Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Mar 20;12(3):734. doi: 10.3390/cancers12030734

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Functional in vitro assays to evaluate the modulation of angiogenesis and endothelial cell permeability by EV treatment. (A–D) Tube formation assay using human umbilical vein endothelial cells (HUVECs) treated with control EVs in H23 (A) and HCC44 (C) and lincRNA-p21-silenced EVs in H23 (B) and HCC44 (D). (E) Quantification of relative number of adherent cells in a cell adhesion assay using a monolayer of HUVECs treated for 12 h with control EVs or lincRNA-p21-silenced EVs. (F,G) A representative image of HCC44 cell adhesion assay. Blue staining using DAPI indicates nucleus and HCC44 cells were stained with cell tracker in red (20× magnification). (H,I) lincRNA-p21 silencing reduced the EV levels of miR-23a, miR-146b, miR-330, and miR-494 in both cell lines. (J,K) EV conditioning increases the expression of miR-23a, miR-146b, miR-330, and miR-494 in HUVECs. (L,M) EV conditioning increases the expression of lincRNA-p21, GLUT1, PFKFB3, and GAPDH in HUVECs. * p < 0.05; ** p < 0.01.