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. 2020 Mar 13;12(3):677. doi: 10.3390/cancers12030677

Figure 3.

Figure 3

Efficacy analysis. Evaluation of the effects of chemotherapy on cancer cell lines (HCT 116, MIA PaCa-2) xenotransplanted in 2 dpf zebrafish embryos. Each embryo was imaged at 2 hpi, 1 dpi, 2 dpi and the relative area is the Dil-stained area normalized with respect to the 2 hpi time point. (A) Chemosensitivity of HCT 116 xenografts, d.f. = 8. A statistically significant increase in relative area was observed in all groups. (B) Chemosensitivity of HCT 116 xenografts, d.f. = 5. A statistically significant increase in the relative area was observed in the control, 5-FU, FOLFOX and FOLFIRI but not in FOLFOXIRI. (C) Chemosensitivity of MIA PaCa-2 xenografts, d.f. = 5. A statistically significant increase in relative area was observed in the control, GEMOX and FOLFOXIRI treatments but not in GEM and GEM/nab-P. Data are mean ± SEM and are representative of three independent assays. N = 15 (embryos), 2-way ANOVA followed by Bonferroni correction (all groups compared against control group). * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.