Figure 4.

D2 and NANOG are co-expressed in the late phases of squamous cell carcinoma (SCC) tumor progression. (A) Schematic representation of the chemical cancerogenesis experiment for SCC tumor progression in mice. (B) Expression profile of D2 mRNA and NANOG mRNA during mouse SCC tumorigenesis. Data represent the mean of three independent experiments performed in triplicate. (C–E) The expression of D2, NANOG, and NANOG-P8 mRNA was measured in human SCC tumors at different pathologic stages in comparison with normal skin counterparts. All samples were run in triplicate and referred to normal skin set arbitrarily as 1. (F,G) Pearson’s correlation analysis was performed for the same data as in C–E. PCR analysis was performed in 20 different tissues for each grading. ** p < 0.01; *** p < 0.001