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. 2020 Mar 16;12(3):699. doi: 10.3390/cancers12030699

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Metastases within the diaphragm. (A) Solid pattern of invasion of PEO4 cells visualized by H&E, and expression of p53, CA125, and human nucleoli marker (HNM). (B) Micropapillary pattern of invasion. (C,D) Different morphological features of metastases by PEO6 cells showing mostly an invasive front with slit-like areas that stain positive for p53, CA125, and HNM. Notice the strongest positivity of the markers in the multicellular structures located around the surface of the diaphragm. Scale bars = 50 μm.