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. 2020 Mar 24;12(3):764. doi: 10.3390/cancers12030764

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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CCNE1 and FOXM1 are overexpressed in GDHO (+) EOC. (a) CCNE1 expression in NO, GDHO (+) EOC, and GDHO (–) EOC, determined by Affymetrix microarray. (b) FOXM1 expression in NO, GDHO (+) EOC, and GDHO (–) EOC, determined by Affymetrix microarray. Means ± SD are plotted, and Mann–Whitney p-values are indicated. (c) FOXM1 mRNA expression vs. LINE-1 methylation in an expanded set of EOC tumor samples. FOXM1 expression was measured by RT-qPCR and LINE-1 methylation was measured by pyrosequencing. Spearman test results and p-value are shown. (d) Western blot analysis of FOXM1 protein expression in NO, GDHO (+) EOC, and GDHO (–) EOC. The upper and lower blots are comprised of different sets of samples. Ponceau S staining is shown as a loading control.