Table 1.

Primer sequences used for quantitative real-time PCR

Gene name	Primer sequence	Number of base pairs	Primer concentration (nM)
ALP
Forward	5′-GTGCCAGAGAAAGAGAGAGAC-3′	21	32.6
Reverse	5′-GACGCCCATACCATCTCC-3′	18	37.1
OCN
Forward	5′-GAGTCTGACAAAGCCTTCA-3′	19	35.4
Reverse	5′-AGCCATACTGGTCTGATAG-3′	19	36.4
COLLI
Forward	5′-ACTGTCCCAACCCCCAAAG-3′	19	33
Reverse	5′-CGTATTCTTCCGGGCAGAAA-3′	20	33.3
GAPDH
Forward	5′-GTCGGTGTGAACGGATTTG-3′	19	33.1
Reverse	5′-GAACATGTAGACCATGTAGTTG-3′	22	34.4