Figure 2.
Function of lncRNA 00312 was detected in RCC cell lines after overexpression. (a) Efficacy of overexpressing lncRNA 00312 was verified via real-time PCR (n = 6). NC: cells transfected empty vector as negative control. (b) lncRNA 00312 inhibited cell proliferation in A498 and ACHN cell lines (n = 6). NC: cells transfected empty vector as negative control. (c) lncRNA 00312 inhibited cell invasion in A498 and ACHN cell lines (n = 6). NC, cells transfected empty vector as negative control. (d, e) lncRNA 00312 promoted cell apoptosis indicated by TUNEL staining (n = 6) and Western blot analysis (n = 3). NC: cells transfected empty vector as negative control. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 versus the respective control.