Figure 3.
Function of RCC cell lines was detected after inhibiting lncRNA 00312. (a) Inhibition of lncRNA 00312 was verified via real-time PCR (n = 6). NC: cells transfected empty vector as negative control. (b) Inhibition of lncRNA 00312 increased cell proliferation in A498 and ACHN cell lines (n = 6). NC: cells transfected empty vector as negative control. (c) Inhibition lncRNA 00312 promoted cell invasion in A498 and ACHN cell lines (n = 6). NC: cells transfected empty vector as negative control. (d, e) Cell apoptosis was decreased after inhibiting lncRNA 00312 indicated by TUNEL staining (n = 6) and Western blot analysis (n = 3). NC: cells transfected empty vector as negative control. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 versus respective control.