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. 2020 Mar 23;2020:5737289. doi: 10.1155/2020/5737289

Figure 6.

Figure 6

lncRNA 00312 performed its function via ASS1. (a) RT-PCR analysis determined efficacy of interfering ASS1 (n = 6). NC: cells transfected two empty vectors as negative control. (b) Interference of ASS1 could increase cell proliferation and inhibited function of lncRNA 00312 (n = 6). NC: cells transfected two empty vectors as negative control. (c, d) Inhibiting ASS1 diminished the effect on promoting apoptosis of lncRNA 00312 and further inhibited cell apoptosis by TUNEL staining (n = 6) and Western blot analysis (n = 3). NC: cells transfected two empty vectors as negative control. (e) ASS1 inhibition increased cell invasion and attenuated the effect of lncRNA 00312 inhibiting cell invasion (n = 6). ∗,$,&P < 0.05, ∗∗,$$,&&P < 0.01, and ∗∗∗,$$$,&&&P < 0.001 versus respective control (multiple comparison: ∗NC group versus lncRNA 00312 group; $NC group versus si-ASS1 group; &lncRNA 00312 group versus lncRNA 00312+si-ASS1 group). NC: cells transfected nonsense sequence as negative control. (f) Western blot analysis result for ASS1 expression. lncRNA 00312+: cells transfected lncRNA 00312 overexpression vector; lncRNA 00312-: cells transfected empty vector of lncRNA 00312 overexpression vector; ASS1 expression +: cells transfected ASS1 overexpression plasmid; ASS1 expression -: cells transfected empty si-ASS1 overexpression plasmid.