. 2020 Apr 7;51:50. doi: 10.1186/s13567-020-00776-y

Table 4.

Oxidative and epigenetic DNA damage and the activities of repair enzymes in the blood and ileal tissues of 42-day-old turkeys fed diets with different arginine (Arg) to lysine (Lys) ratios and a low methionine (Met) level, challenged withClostridium perfringens—(Experiment 1).

Item	Ileal tissue					Blood
Item	8-OHdG pg/µL DNA	Methylation DNA, %	APE-1 ng/g	TDG ng/g	ANPG ng/g	8-OHdG pg/mL	Methylation DNA, %	APE-1 ng/mL	TDG ng/mL	ANPG ng/mL
Arg₉₀*Infection (−)	24.26^d	14.92^c	1731.0^a	1056.9	39.01	4.424^c	28.66	160.1	83.68	4.197
Arg₉₀*Infection (+)	53.44^b	18.72^ab	933.2^c	596.3	17.78	6.825^a	58.23	146.7	83.99	4.361
Arg₁₀₀*Infection (−)	28.04^d	15.37^bc	1746.2^a	994.5	42.41	5.061^bc	30.14	182.5	100.30	4.747
Arg₁₀₀*Infection (+)	86.21^a	19.55^a	995.3^c	658.9	21.07	6.031^ab	46.46	152.2	92.31	4.601
Arg₁₁₀*Infection (−)	38.08^c	19.00^ab	1382.5^b	876.3	37.38	5.711^abc	30.20	147.4	94.87	4.772
Arg₁₁₀*Infection (+)	84.96^a	13.21^c	1018.2^c	651.0	23.16	6.017^ab	52.06	144.8	93.37	4.978
SEM	3.757	0.493	57.80	33.85	1.673	0.175	2.252	3.025	1.607	0.075
Arg level, %
90	38.85^b	16.82	1332.1	826.6	28.39	5.624	43.44	153.4^ab	83.83^b	4.279^b
100	57.12^a	17.46	1370.7	826.7	31.74	5.546	38.30	167.4^a	96.31^a	4.674^ab
110	59.96^a	16.11	1200.3	763.6	30.27	5.864	41.13	146.1^b	94.12^a	4.875^a
C. perfringens infection¹
–	30.13^b	16.43	1619.9^a	975.9^a	39.60^a	5.065^b	29.67^b	163.3^a	92.95	4.572
+	74.43^a	17.16	982.2^b	635.4^b	20.67^b	6.291^a	52.25^a	147.9^b	89.89	4.647
P value
Arg	0.001	0.329	0.075	0.433	0.356	0.641	0.399	0.004	0.002	0.003
Infection	0.001	0.327	0.001	0.001	0.001	0.001	0.001	0.003	0.292	0.589
Arg × infection	0.001	0.001	0.012	0.119	0.222	0.014	0.221	0.084	0.468	0.523

Diets contained three levels of Arg relative to Lys (90, 100 and 110%; Arg₉₀, Arg₁₀₀ and Arg₁₁₀, respectively) and a low level of Met (30% dietary Lys).

¹At 34, 36 and 37 days of age, birds were infected with 1 mL (per os directly into the crop) of a culture medium of C. perfringens type A strain 56 containing approximately 10⁸ CFU/mL of bacteria. ^a,b,cMeans within a row with different superscripts differ significantly (P < 0.05).