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. 2020 Apr 7;51:50. doi: 10.1186/s13567-020-00776-y

Table 6.

Gene expression in the ileal tissues of 42-day-old turkeys fed diets with different arginine (Arg) to lysine (Lys) ratios and a high methionine (Met) level, challenged withClostridium perfringens—(Experiment 2).

Item ZO-1 Occludin GLP2 OGG1 TFF2
Arg90*Infection (−) 0.794 0.005 0.375 0.184 0.003
Arg90*Infection (+) 1.918 0.022 1.145 0.666 0.011
Arg100*Infection (−) 0.841 0.006 0.385 0.172 0.003
Arg100*Infection (+) 2.485 0.014 1.590 0.545 0.009
Arg110*Infection (−) 0.838 0.005 0.368 0.283 0.003
Arg110*Infection (+) 2.253 0.014 1.219 0.616 0.007
SEM 0.120 0.001 0.091 0.044 0.0007
Arg level,  %
 90 1.356 0.013 0.760 0.425 0.007
 100 1.663 0.010 0.987 0.359 0.006
 110 1.545 0.009 0.793 0.450 0.005
C. perfringens infection1
 – 0.824b 0.005b 0.376b 0.213b 0.003b
 + 2.219a 0.017a 1.318a 0.609a 0.009a
P value
 Arg 0.134 0.144 0.259 0.532 0.421
 infection 0.001 0.001 0.001 0.001 0.001
 Arg × infection 0.237 0.078 0.301 0.654 0.283

Diets contained three levels of Arg relative to Lys (90, 100 and 110%; Arg90, Arg100 and Arg110, respectively) and a high level of Met (45% dietary Lys).

1At 34, 36 and 37 days of age, birds were infected with 1 mL (per os directly into the crop) of a culture medium of C. perfringens type A strain 56 containing approximately 108 CFU/mL of bacteria. a,bMeans within a row with different superscripts differ significantly (P < 0.05).