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. 2020 Apr 8;27:50. doi: 10.1186/s12929-020-00641-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — The frequency of Tfh cells and IL-21 producing Tfh cells in patients with acute dengue in the acute phase. The expression of total Tfh cells (cells expressing CD4 + CXCR5+) and IL-21 producing Tfh cells in two patients; patient a with acute dengue infection (day 7 since onset of illness) and patient b with acute dengue infection (day 6 since onset of illness) are shown. The frequency of Tfh cells (c) was assessed in patients with acute DENV2 infection (n = 40), in healthy individuals (n = 12), in patients with DF (n = 18), in patients with DHF (n = 22), in patients with primary infection (n = 13) and secondary dengue infection (n = 27) by multiparametric flowcytometry. The lines display the median and the IQR. **P < 0.01, ***p < 0.001, ****p < 0.0001