Table 1.
Results of Toxoplasma gondii oocyst detection and quantification in the collected vegetables and fruits
| Sample number | Product | Weight (g) | Origin | Collection date | Agricultural system | Product presentation | DNAa | Enzymatic restriction confirmation | Sequence confirmation | Mean no. of oocysts/g |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Lettuce | 3600 | Aguçadoura (Póvoa do Varzim) | 12-Jul-2018 | Conventional | Bulk | Positive | Yes | Yes | 1.3 |
| 2 | Carrot | 924 | Aguçadoura (Póvoa do Varzim) | 12-Jul-2018 | Conventional | Bulk | Negative | – | – | – |
| 3 | Parsley | 266 | Aguçadoura (Póvoa do Varzim) | 12-Jul-2018 | Conventional | Bulk | Negative | – | – | – |
| 4 | Lettuce | 261 | Aguçadoura (Póvoa do Varzim) | 10-Aug-2018 | Conventional | Bulk | Negative | – | – | – |
| 5 | Lettuce | 261 | Mabegondo-Abegondo (Galiza) | 13-Aug-2018 | Organic | Bulk | Positive | Yes | Yes | 3.6 |
| 6 | Lettuce | 600 | Gondomar (Porto) | 13-Aug-2018 | Organic | Bulk | Positive | Yes | Yes | 2.8 |
| 7 | Strawberry | 64 | São Félix da Marinha (Vila Nova de Gaia) | 05-Sep-2018 | Organic | Bulk | Positive | Yes | Yes | 8.6 |
| 8 | Watercress | 792 | Boavista dos Pinheiros (Odemira) | 27-Sep-2018 | Conventional | Bulk | Positive | Yes | Yes | 0.6 |
| 9 | Lettuce | 1246 | Boavista dos Pinheiros (Odemira) | 27-Sep-2018 | Conventional | Bulk | Positive | Yes | Yes | 1.1 |
| 10 | Lettuce | 2031 | Boavista dos Pinheiros (Odemira) | 27-Sep-2018 | Conventional | Bulk | Negative | – | – | – |
| 11 | Coriander | 749 | Boavista dos Pinheiros (Odemira) | 27-Sep-2018 | Conventional | Bulk | Negative | – | – | – |
| 12 | Parsley | 632 | Boavista dos Pinheiros (Odemira) | 27-Sep-2018 | Conventional | Bulk | Negative | – | – | – |
| 13 | Watercress | 900 | Boavista dos Pinheiros (Odemira) | 02-Oct-2018 | Conventional | RTE | Negative | – | – | – |
| 14 | Mix salad | 800 | Boavista dos Pinheiros (Odemira) | 02-Oct-2018 | Conventional | RTE | Negative | – | – | – |
| 15 | Coriander | 450 | Boavista dos Pinheiros (Odemira) | 02-Oct-2018 | Conventional | Packaged | Positive | Yes | Yes | 3.9 |
| 16 | Parsley | 450 | Boavista dos Pinheiros (Odemira) | 02-Oct-2018 | Conventional | Packaged | Positive | Yes | Yes | 44.5 |
| 17 | Watercress | 900 | Boavista dos Pinheiros (Odemira) | 12-Oct-2018 | Conventional | RTE | Positive | Yes | Yes | 3.2 |
| 18 | Mixed salad | 700 | Boavista dos Pinheiros (Odemira) | 12-Oct-2018 | Conventional | RTE | Positive | Yes | Yes | 6.0 |
| 19 | Coriander | 350 | Boavista dos Pinheiros (Odemira) | 12-Oct-2018 | Conventional | Packaged | Positive | Yes | Yes | 38.3 |
| 20 | Parsley | 350 | Boavista dos Pinheiros (Odemira) | 12-Oct-2018 | Conventional | Packaged | Negative | – | – | – |
| 21 | Mixed salad | 400 | Boavista dos Pinheiros (Odemira) | 04-Feb-2019 | Conventional | RTE | Positive | Yes | Yes | 179.9 |
| 22 | Lettuce | 494 | Torre de Bera (Coimbra) | 04-Mar-2019 | Organic | Bulk | Negative | – | – | – |
| 23 | Lettuce | 1100 | Torre de Bera (Coimbra) | 04-Mar-2019 | Organic | Bulk | Negative | – | – | – |
| 24 | Strawberry | 500 | Macedo de Cavaleiros (Bragança) | 27-May-2019 | Conventional | Bulk | Positive | Yes | Yes | 2.5 |
| 25 | Strawberry | 500 | Macedo de Cavaleiros (Bragança) | 27-May-2019 | Conventional | Bulk | Positive | Yes | Yes | 3.1 |
| 26 | Mixed salad | 800 | Milagro (Navarra) | 17-Jun-2019 | Conventional | RTE | Negative | – | – | – |
| 27 | Mixed salad | 800 | Milagro (Navarra) | 17-Jun-2019 | Conventional | RTE | Negative | – | – | – |
| 28 | Arugula | 500 | Milagro (Navarra) | 17-Jun-2019 | Conventional | RTE | Negative | – | – | – |
| 29 | Raspberry | 500 | Brejão (Odemira) | 17-Jun-2019 | Conventional | Packaged | Negative | – | – | – |
| 30 | Blueberry | 500 | Laundos (Póvoa do Varzim) | 17-Jun-2019 | Conventional | Packaged | Negative | – | – | – |
| 31 | Lettuce | 250 | São Pedro da Cova (Gondomar) | 18-Jun-2019 | Organic | Bulk | Negative | – | – | – |
| 32 | Lettuce | 270 | São Pedro da Cova (Gondomar) | 18-Jun-2019 | Organic | Bulk | Positive | Yes | Yes | 53.3 |
| 33 | Mixed salad | 800 | Milagro (Navarra) | 15-Jul-2019 | Conventional | RTE | Negative | – | – | – |
| 34 | Blueberry | 625 | Santa Leucádia (Baião) | 15-Jul-2019 | Organic | Packaged | Negative | – | – | – |
| 35 | Raspberry | 625 | Santa Leucádia (Baião) | 15-Jul-2019 | Conventional | Packaged | Negative | – | – | – |
a529-bp REP
Abbreviations: REP: repetitive region; RTE: ready-to-eat