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Mutant and recombinant RNA2s analysis in radish by tissue immuno-blotting assay
| Inoculum | Inoculated leavesa | Upper leavesb |
|---|---|---|
| Mock | 0/8c | 0/8c |
| Y1 D2(D2a-C/D2bstop) D3 | 8/8 | 0/8 |
| Y1 Y2(∆Y2a-C/D2b) D3 | 8/8 | 0/8 |
| Y1 D2(D2a-C/∆D2b-C) D3 | 8/8 | 0/8 |
| Y1 Y2(D2a-C/D2b) D3 | 8/8 | 8/8 |
| Y1 Y2(D2b-C) D3 | 8/8 | 0/8 |
Data were recorded at a10 dpi; b21 dpi; cNumber of infected plants/number of inoculated plants. Some samples with very low levels of viral accumulation were included as positive
