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. 2020 Mar 4;9(3):619. doi: 10.3390/cells9030619

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Live cell images of the temporal differentiation of human adipose derived stem cells (ADSCs) induced with 0.2 mM valproic acid (VPA) at (A) 0 h, (B) 3 h, (C) 6 h, and (D) 24 h at 10× magnification. The cellular morphology changes rapidly through the time points, with cells adopting a more slender and bipolar orientation with neurite extensions (arrows). (E) Average cell count across the treatments including all controls with standard error bars. Relatively minimal changes in numbers are shown across all treatments. A Student’s t-test revealed no significant change in cell numbers.

Live cell images of the temporal differentiation of human adipose derived stem cells (ADSCs) induced with 0.2 mM valproic acid (VPA) at (A) 0 h, (B) 3 h, (C) 6 h, and (D) 24 h at 10× magnification. The cellular morphology changes rapidly through the time points, with cells adopting a more slender and bipolar orientation with neurite extensions (arrows). (E) Average cell count across the treatments including all controls with standard error bars. Relatively minimal changes in numbers are shown across all treatments. A Student’s t-test revealed no significant change in cell numbers.