Skip to main content
. 2020 Feb 26;9(3):546. doi: 10.3390/cells9030546
AF594 Alexa Fluor 594
α-GalT α-1,3-galactosyltransferase
CRISPR/Cas9 Clustered regularly interspaced palindrome repeats (CRISPR)/Caspase 9 (Cas9)
crRNA CRISPR RNA
EP Electroporation
FBS Fetal bovine serum
FITC Fluorescein isothiocyanate
gRNA Guide RNA
GOI Gene of interest
HDR Homology direct repair
IB4 Isolectin BS-I-B4 lectin
i-GONAD Improved genome-editing via oviductal nucleic acid delivery
indels Insertion and deletion mutations
IP Intraperitoneally
IVF In vitro fertilization
KI Knock-in
LD Large deletion
NHEJ Non-homologous end joining
ODNs Oligodeoxynucleotides
PAM Protospacer adjacent motif
PBS Dulbecco’s modified Ca2+, Mg2+-free phosphate-buffered saline
PCR Polymerase chain reaction
Pp Poring pulse
rAAV-6 Recombinant adeno-associated virus serotype 6
RNP Ribonucleoprotein
SCNT Somatic cell nuclear transfer
si-GONAD Sequential i-GONAD
simi-GONAD Simultaneous i-GONAD
ss Single-stranded
Tp Transfer pulse
tracrRNA Trans-activating small RNA
WGA Whole genome amplification
ZP Zona pellucida