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. 2020 Mar 6;9(3):637. doi: 10.3390/cells9030637

Figure 2.

Figure 2

Effects of NOX5-β overexpression on prostaglandin (PG) biosynthesis in TeloHAEC. (a) COX-2 mRNA levels in NOX5-β- and GFP-infected cells for 12 h, in the presence or absence of ML090. n = 6. *** p < 0.001 vs. GFP, **** p < 0.0001 vs. GFP, and #### p < 0.0001 vs. NOX5. (b) Representative COX-2 and β-actin immunoblots from cells infected for 12 h, and quantification of the COX-2 protein. n = 5. * p < 0.05 vs. GFP. (c) COX-2 promoter transcriptional activity of cells infected for 24 h. n = 6. * p < 0.05 vs. GFP. (d) PGE2 levels present in cell supernatant after 12 h of infection in the presence or absence of ML090. n = 6. *** p < 0.001 vs. GFP, and ## p < 0.01 vs. NOX5.