Table 1.
Primer sequences for quantitative real-time PCR analysis.
| Gene | Forward Sequence | Reverse Sequence |
|---|---|---|
| Hs cPLA-2 | GAAGATTCTCAGGTTTTAAAGACGC | GGCATCCATTAACGTAATCTCCAA |
| Hs COX-1 | AAGTACCAGGTGCTGGATG | TGATGGTCTCCCCTATGA |
| Hs COX-2 | TGCGGGAACACAACAGAGT | TAGCCACTCAAGTGTTGCAC |
| Hs PGES | AGGATGCCCTGAGACACGGAG | CCCAGGAAAAGGAAGGGGTAG |
| Hs PTGIS | ACATCTTTACTATACTGGTTGGGGG | TGTGGAGAAGAGTCAGTTTCATC |
| Hs NOX1 | GCAGGGAGACAGGTGCCTTTTCC | CTACAGACTTGGGGTGGGAGGT |
| Hs NOX2 | TTCCAGTGCGTGCTGCTCA | CTGCGGTCTGCCCACGTAC |
| Hs NOX4 | CTGGCTCGCCAACGAAGGGG | GCTTGGAACCTTCTGTGATCCTCGG |
| Hs NOX5 | ATGAGTGGCACCCCTTCACCATCAG | TCAGCAGGCTCACAAACCACTCGAA |
| Hs GAPDH | CCAAGGTCATCCATGACAAC | TGTCATACCAGGAAATGAGC |
| Mm cPLA-2 | ACGTGATGTGCCGGTGG | AAGAGAGGCAAAGGACACCG |
| Mm COX-1 | ACTCACAGTGCGGTCCAAC | AACTCCCTTCTCAGCAGCAG |
| Mm COX-2 | TTCGGGAGCACAACAGAGT | TAACCGCTCAGGTGTTGCAC |
| Mm PGES | AGGATGCGCTGAAACGTGGAG | CCGAGGAAGAGGAAAGGATAG |
| Mm PTGIS | TTGTCAGCGGGGGATAAA | GACCCATATTCCCCTGTGTG |
| Mm TXA2S | AACAGAATGGCCTCAGGTCT | AGTTCACAGGCTTGGCTGAT |
| Mm NOX2 | ACTCCTTGGGTCAGCACTGG | GTTCCTGTCCAGTTGTCTTCG |
| Mm NOX4 | GGAGACTGGACAGAACGATTCC | TGTATAACTTAGGGTAATTTCTAGAGTGAATGA |
| Mm GAPDH | TGCTGAGTATGTCGTGGAGTCTA | CATTGCTGACAATCTTGAGTGAG |
Hs, Homo sapiens; Mm, Mus musculus; c-PLA-2, cytosolic phospholipase A2; COX, cyclooxygenase; NOX, NADPH oxidase; PGES, prostaglandin E synthase; PTGIS, prostaglandin I2 synthase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; TXA2S, thromboxane A2 synthase.