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. 2020 Mar 2;9(3):594. doi: 10.3390/cells9030594

Figure 4.

Figure 4

Src and Akt phosphorylation are involved in APLN-induced VEGF synthesis. (A,B) OASFs were pretreated with Src/Akt inhibitors for 30 min or Src/Akt siRNAs for 24 h, then incubated with APLN for 24 h. (A) VEGF mRNA levels were examined using RT-qPCR (n = 4), and (B) excreted VEGF protein levels were quantified using ELISA assay (n = 5). (C,D) After pretreatment with Src/Akt inhibitors or Src/Akt siRNA and incubation with APLN, the culture medium was collected and subjected to EPC (C) tube formation (n = 5) and (D) migration assays (n = 5). (E) OASFs were incubated with APLN, and the extent of Src/Akt phosphorylation was examined using Western blot analysis (n = 3). (F) OASFs pretreated with a FAK inhibitor before APLN incubation were assessed for the extent of Src/Akt phosphorylation, while OASFs pretreated with a Src inhibitor before APLN incubation were assessed for Akt phosphorylation (n = 3). * p < 0.05 compared with control; # p < 0.05 compared with the APLN-treated group.