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. 2020 Mar 2;9(3):594. doi: 10.3390/cells9030594

Figure 5.

Figure 5

Apelin suppression of miR-150-5p enhances VEGF production. (A) Open-source software (TargetScan, miRMap, RNAhybrid, and miRWalk) was employed to determine which miRNAs could potentially inhibit VEGF transcription. (B) OASFs were incubated with APLN. miR-150-5p expression levels were examined using RT-qPCR assay (n = 4). (C,D) OASFs were transfected with miR-150-5p mimic and then stimulated by APLN. (C) VEGF transcription and (D) translation levels were examined using RT-qPCR (n = 4) and ELISA assays (n = 5). (E) OASFs were transfected with the mt-VEGF-3′UTR plasmid with or without miRNA-150-5p mimic, then incubated under APLN. Relative luciferase activity reflected VEGF promoter activity (n = 6). (F) OASFs were treated with FAK, Src, or Akt inhibitor then incubated with APLN. miR-150-5p expression levels were examined using RT-qPCR assay (n = 4). * p < 0.05 compared with control; # p < 0.05 compared with the APLN-treated group.