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. 2020 Mar 3;9(3):603. doi: 10.3390/cells9030603

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Depletion of PPE51 does not affect the growth of Mtb Growth kinetics (A) of the wild-type (black) strain and the T-6/2 (red), PPE51 CRISPR-weak (green) and PPE51 CRISPR-strong (blue) mutants of M. tuberculosis in 7H9/OADC medium, supplemented (dashed lines) or not (solid lines) with aTc. (B) Immunodetection of PPE51 and control SigA proteins in the cell lysis of wild-type M. tuberculosis (lines 1–2) and CRISPR mutants (CRISPR-weak, lines 3–4; CRISPR-strong, lines 5–6) grown in rich medium supplemented or not with aTc, with rabbit anti-PPE51 (upper panel) and anti-SigA (bottom panel) serum. (C) Densitometry analysis of bands representing PPE51 normalized to the control SigA protein.